LOCUS       NC_000913            5040 bp       DNA     linear   BCT 17-MAY-2008
DEFINITION  Escherichia coli str. K-12 substr. MG1655, first 5040 bp
ACCESSION   NC_000913
VERSION     NC_000913.2  GI:49175990
PROJECT     GenomeProject:225
KEYWORDS    .
SOURCE      Escherichia coli str. K12 substr. MG1655
  ORGANISM  Escherichia coli str. K12 substr. MG1655
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Escherichia.
COMMENT     First 5040 from NC_000913.2
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Escherichia coli str. K12 substr. MG1655"
                     /mol_type="genomic DNA"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     gene            190..255
                     /gene="thrL"
                     /locus_tag="b0001"
                     /synonym="ECK0001, JW4367"
                     /db_xref="ECOCYC:EG11277"
                     /db_xref="EcoGene:EG11277"
                     /db_xref="GeneID:944742"
     CDS             190..255
                     /gene="thrL"
                     /locus_tag="b0001"
                     /function="leader; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /GO_process="threonine biosynthetic process"
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="NP_414542.1"
                     /db_xref="GI:16127995"
                     /db_xref="ASAP:ABE-0000006"
                     /db_xref="UniProtKB/Swiss-Prot:P0AD86"
                     /db_xref="ECOCYC:EG11277"
                     /db_xref="EcoGene:EG11277"
                     /db_xref="GeneID:944742"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            337..2799
                     /gene="thrA"
                     /locus_tag="b0002"
                     /synonym="ECK0002, thrD, JW0001, Hs, thrA2, thrA1"
                     /db_xref="ECOCYC:EG10998"
                     /db_xref="EcoGene:EG10998"
                     /db_xref="GeneID:945803"
     CDS             337..2799
                     /gene="thrA"
                     /locus_tag="b0002"
                     /EC_number="2.7.2.4"
                     /EC_number="1.1.1.3"
                     /function="enzyme; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /function="1.5.1.21 metabolism; building block
                     biosynthesis; amino acids; homoserine"
                     /function="7.1 location of gene products; cytoplasm"
                     /experiment="N-terminus verified by Edman degradation:
                     PMID 354697,4562989"
                     /GO_component="cytoplasm"
                     /GO_process="homoserine biosynthetic process; methionine
                     biosynthetic process; threonine biosynthetic process"
                     /note="bifunctional: aspartokinase I (N-terminal);
                     homoserine dehydrogenase I (C-terminal)"
                     /codon_start=1
                     /transl_table=11
                     /product="fused aspartokinase I and homoserine
                     dehydrogenase I"
                     /protein_id="NP_414543.1"
                     /db_xref="GI:16127996"
                     /db_xref="ASAP:ABE-0000008"
                     /db_xref="UniProtKB/Swiss-Prot:P00561"
                     /db_xref="ECOCYC:EG10998"
                     /db_xref="EcoGene:EG10998"
                     /db_xref="GeneID:945803"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            2801..3733
                     /gene="thrB"
                     /locus_tag="b0003"
                     /synonym="ECK0003, JW0002"
                     /db_xref="ECOCYC:EG10999"
                     /db_xref="EcoGene:EG10999"
                     /db_xref="GeneID:947498"
     CDS             2801..3733
                     /gene="thrB"
                     /locus_tag="b0003"
                     /EC_number="2.7.1.39"
                     /function="enzyme; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /function="7.1 location of gene products; cytoplasm"
                     /GO_component="cytoplasm"
                     /GO_process="threonine biosynthetic process"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="NP_414544.1"
                     /db_xref="GI:16127997"
                     /db_xref="ASAP:ABE-0000010"
                     /db_xref="UniProtKB/Swiss-Prot:P00547"
                     /db_xref="ECOCYC:EG10999"
                     /db_xref="EcoGene:EG10999"
                     /db_xref="GeneID:947498"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPETA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            3734..5020
                     /gene="thrC"
                     /locus_tag="b0004"
                     /synonym="ECK0004, JW0003"
                     /db_xref="ECOCYC:EG11000"
                     /db_xref="EcoGene:EG11000"
                     /db_xref="GeneID:945198"
     CDS             3734..5020
                     /gene="thrC"
                     /locus_tag="b0004"
                     /EC_number="4.2.3.1"
                     /function="enzyme; Amino acid biosynthesis: Threonine"
                     /function="1.5.1.8 metabolism; building block
                     biosynthesis; amino acids; threonine"
                     /function="7.1 location of gene products; cytoplasm"
                     /experiment="N-terminus verified by Edman degradation:
                     PMID 9298646,9600841,9740056"
                     /GO_component="cytoplasm"
                     /GO_process="threonine biosynthetic process"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="NP_414545.1"
                     /db_xref="GI:16127998"
                     /db_xref="ASAP:ABE-0000012"
                     /db_xref="UniProtKB/Swiss-Prot:P00934"
                     /db_xref="ECOCYC:EG11000"
                     /db_xref="EcoGene:EG11000"
                     /db_xref="GeneID:945198"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQETRNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
ORIGIN      
        1 agcttttcat tctgactgca acgggcaata tgtctctgtg tggattaaaa aaagagtgtc
       61 tgatagcagc ttctgaactg gttacctgcc gtgagtaaat taaaatttta ttgacttagg
      121 tcactaaata ctttaaccaa tataggcata gcgcacagac agataaaaat tacagagtac
      181 acaacatcca tgaaacgcat tagcaccacc attaccacca ccatcaccat taccacaggt
      241 aacggtgcgg gctgacgcgt acaggaaaca cagaaaaaag cccgcacctg acagtgcggg
      301 cttttttttt cgaccaaagg taacgaggta acaaccatgc gagtgttgaa gttcggcggt
      361 acatcagtgg caaatgcaga acgttttctg cgtgttgccg atattctgga aagcaatgcc
      421 aggcaggggc aggtggccac cgtcctctct gcccccgcca aaatcaccaa ccacctggtg
      481 gcgatgattg aaaaaaccat tagcggccag gatgctttac ccaatatcag cgatgccgaa
      541 cgtatttttg ccgaactttt gacgggactc gccgccgccc agccggggtt cccgctggcg
      601 caattgaaaa ctttcgtcga tcaggaattt gcccaaataa aacatgtcct gcatggcatt
      661 agtttgttgg ggcagtgccc ggatagcatc aacgctgcgc tgatttgccg tggcgagaaa
      721 atgtcgatcg ccattatggc cggcgtatta gaagcgcgcg gtcacaacgt tactgttatc
      781 gatccggtcg aaaaactgct ggcagtgggg cattacctcg aatctaccgt cgatattgct
      841 gagtccaccc gccgtattgc ggcaagccgc attccggctg atcacatggt gctgatggca
      901 ggtttcaccg ccggtaatga aaaaggcgaa ctggtggtgc ttggacgcaa cggttccgac
      961 tactctgctg cggtgctggc tgcctgttta cgcgccgatt gttgcgagat ttggacggac
     1021 gttgacgggg tctatacctg cgacccgcgt caggtgcccg atgcgaggtt gttgaagtcg
     1081 atgtcctacc aggaagcgat ggagctttcc tacttcggcg ctaaagttct tcacccccgc
     1141 accattaccc ccatcgccca gttccagatc ccttgcctga ttaaaaatac cggaaatcct
     1201 caagcaccag gtacgctcat tggtgccagc cgtgatgaag acgaattacc ggtcaagggc
     1261 atttccaatc tgaataacat ggcaatgttc agcgtttctg gtccggggat gaaagggatg
     1321 gtcggcatgg cggcgcgcgt ctttgcagcg atgtcacgcg cccgtatttc cgtggtgctg
     1381 attacgcaat catcttccga atacagcatc agtttctgcg ttccacaaag cgactgtgtg
     1441 cgagctgaac gggcaatgca ggaagagttc tacctggaac tgaaagaagg cttactggag
     1501 ccgctggcag tgacggaacg gctggccatt atctcggtgg taggtgatgg tatgcgcacc
     1561 ttgcgtggga tctcggcgaa attctttgcc gcactggccc gcgccaatat caacattgtc
     1621 gccattgctc agggatcttc tgaacgctca atctctgtcg tggtaaataa cgatgatgcg
     1681 accactggcg tgcgcgttac tcatcagatg ctgttcaata ccgatcaggt tatcgaagtg
     1741 tttgtgattg gcgtcggtgg cgttggcggt gcgctgctgg agcaactgaa gcgtcagcaa
     1801 agctggctga agaataaaca tatcgactta cgtgtctgcg gtgttgccaa ctcgaaggct
     1861 ctgctcacca atgtacatgg ccttaatctg gaaaactggc aggaagaact ggcgcaagcc
     1921 aaagagccgt ttaatctcgg gcgcttaatt cgcctcgtga aagaatatca tctgctgaac
     1981 ccggtcattg ttgactgcac ttccagccag gcagtggcgg atcaatatgc cgacttcctg
     2041 cgcgaaggtt tccacgttgt cacgccgaac aaaaaggcca acacctcgtc gatggattac
     2101 taccatcagt tgcgttatgc ggcggaaaaa tcgcggcgta aattcctcta tgacaccaac
     2161 gttggggctg gattaccggt tattgagaac ctgcaaaatc tgctcaatgc aggtgatgaa
     2221 ttgatgaagt tctccggcat tctttctggt tcgctttctt atatcttcgg caagttagac
     2281 gaaggcatga gtttctccga ggcgaccacg ctggcgcggg aaatgggtta taccgaaccg
     2341 gacccgcgag atgatctttc tggtatggat gtggcgcgta aactattgat tctcgctcgt
     2401 gaaacgggac gtgaactgga gctggcggat attgaaattg aacctgtgct gcccgcagag
     2461 tttaacgccg agggtgatgt tgccgctttt atggcgaatc tgtcacaact cgacgatctc
     2521 tttgccgcgc gcgtggcgaa ggcccgtgat gaaggaaaag ttttgcgcta tgttggcaat
     2581 attgatgaag atggcgtctg ccgcgtgaag attgccgaag tggatggtaa tgatccgctg
     2641 ttcaaagtga aaaatggcga aaacgccctg gccttctata gccactatta tcagccgctg
     2701 ccgttggtac tgcgcggata tggtgcgggc aatgacgtta cagctgccgg tgtctttgct
     2761 gatctgctac gtaccctctc atggaagtta ggagtctgac atggttaaag tttatgcccc
     2821 ggcttccagt gccaatatga gcgtcgggtt tgatgtgctc ggggcggcgg tgacacctgt
     2881 tgatggtgca ttgctcggag atgtagtcac ggttgaggcg gcagagacat tcagtctcaa
     2941 caacctcgga cgctttgccg ataagctgcc gtcagaacca cgggaaaata tcgtttatca
     3001 gtgctgggag cgtttttgcc aggaactggg taagcaaatt ccagtggcga tgaccctgga
     3061 aaagaatatg ccgatcggtt cgggcttagg ctccagtgcc tgttcggtgg tcgcggcgct
     3121 gatggcgatg aatgaacact gcggcaagcc gcttaatgac actcgtttgc tggctttgat
     3181 gggcgagctg gaaggccgta tctccggcag cattcattac gacaacgtgg caccgtgttt
     3241 tctcggtggt atgcagttga tgatcgaaga aaacgacatc atcagccagc aagtgccagg
     3301 gtttgatgag tggctgtggg tgctggcgta tccggggatt aaagtctcga cggcagaagc
     3361 cagggctatt ttaccggcgc agtatcgccg ccaggattgc attgcgcacg ggcgacatct
     3421 ggcaggcttc attcacgcct gctattcccg tcagcctgag cttgccgcga agctgatgaa
     3481 agatgttatc gctgaaccct accgtgaacg gttactgcca ggcttccggc aggcgcggca
     3541 ggcggtcgcg gaaatcggcg cggtagcgag cggtatctcc ggctccggcc cgaccttgtt
     3601 cgctctgtgt gacaagccgg aaaccgccca gcgcgttgcc gactggttgg gtaagaacta
     3661 cctgcaaaat caggaaggtt ttgttcatat ttgccggctg gatacggcgg gcgcacgagt
     3721 actggaaaac taaatgaaac tctacaatct gaaagatcac aacgagcagg tcagctttgc
     3781 gcaagccgta acccaggggt tgggcaaaaa tcaggggctg ttttttccgc acgacctgcc
     3841 ggaattcagc ctgactgaaa ttgatgagat gctgaagctg gattttgtca cccgcagtgc
     3901 gaagatcctc tcggcgttta ttggtgatga aatcccacag gaaatcctgg aagagcgcgt
     3961 gcgcgcggcg tttgccttcc cggctccggt cgccaatgtt gaaagcgatg tcggttgtct
     4021 ggaattgttc cacgggccaa cgctggcatt taaagatttc ggcggtcgct ttatggcaca
     4081 aatgctgacc catattgcgg gtgataagcc agtgaccatt ctgaccgcga cctccggtga
     4141 taccggagcg gcagtggctc atgctttcta cggtttaccg aatgtgaaag tggttatcct
     4201 ctatccacga ggcaaaatca gtccactgca agaaaaactg ttctgtacat tgggcggcaa
     4261 tatcgaaact gttgccatcg acggcgattt cgatgcctgt caggcgctgg tgaagcaggc
     4321 gtttgatgat gaagaactga aagtggcgct agggttaaac tcggctaact cgattaacat
     4381 cagccgtttg ctggcgcaga tttgctacta ctttgaagct gttgcgcagc tgccgcagga
     4441 gacgcgcaac cagctggttg tctcggtgcc aagcggaaac ttcggcgatt tgacggcggg
     4501 tctgctggcg aagtcactcg gtctgccggt gaaacgtttt attgctgcga ccaacgtgaa
     4561 cgataccgtg ccacgtttcc tgcacgacgg tcagtggtca cccaaagcga ctcaggcgac
     4621 gttatccaac gcgatggacg tgagtcagcc gaacaactgg ccgcgtgtgg aagagttgtt
     4681 ccgccgcaaa atctggcaac tgaaagagct gggttatgca gccgtggatg atgaaaccac
     4741 gcaacagaca atgcgtgagt taaaagaact gggctacact tcggagccgc acgctgccgt
     4801 agcttatcgt gcgctgcgtg atcagttgaa tccaggcgaa tatggcttgt tcctcggcac
     4861 cgcgcatccg gcgaaattta aagagagcgt ggaagcgatt ctcggtgaaa cgttggatct
     4921 gccaaaagag ctggcagaac gtgctgattt acccttgctt tcacataatc tgcccgccga
     4981 ttttgctgcg ttgcgtaaat tgatgatgaa tcatcagtaa aatctattca ttatctcaat
//

